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Sci. Lett. J. 2015, 4: 173
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Review Article
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Full Text
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Measuring equilibrium ultrafast structural and conformational dynamics in bio-molecules: a two dimensional infrared (2D-IR) spectroscopic approach
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Somnath M. Kashid, Pranab Deb, Tapas Haldar, Partha Pratim Roy, Sayan Bagchi
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Physical Chemistry Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, Maharashtra, India
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Abstract |
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In the recent years, 2D-IR spectroscopy has evolved as one of the ideal experimental techniques to study ultrafast structural and conformational dynamics in proteins and enzymes. This article reviews the 2D-IR experimental methodology and provides a qualitative understanding of the spectral signatures of the various ultrafast dynamic processes in the 2D-IR spectrum. The recent literature relating to the use of the 2D-IR spectroscopic technique on biological macromolecules to estimate the timescales of ultrafast structural motions and to understand the connection of the ultrafast dynamics towards their function has been discussed. The future scope of using this experimental technique in a multi-disciplinary approach would provide molecular level understanding of the structure – dynamics – function relationship of bio-molecules.
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Keywords
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2D-IR; Ultrafast spectroscopy; Ultrafast dynamics; Spectral diffusion; Chemical exchange; Proteins and enzymes; Biological chemistry; Biophysics; Molecular dynamics
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