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Sci. Lett. J. 2015, 4: 76
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Research Article
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Full Text
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A label free impedemetric immunosensor for detection of Escherichia coli in water
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Geetesh K Mishraa,#, Gautam Bachera,b,#, Utpal Royc, Sunil Bhanda
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a Biosensor Lab, Department of Chemistry, BITS, Pilani-K.K. Birla Goa Campus, Goa 403726, India
b Department of EEE & I, BITS, Pilani-K.K. Birla Goa Campus, Goa 403726, India
c Department of Biological Sciences, BITS, Pilani-K.K. Birla Goa Campus, Goa 403726, India
# Authors with equal contribution
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Abstract |
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In the presented work, a simple and sensitive impedimetric immunosensor based on silver (Ag) wire electrode for the detection of Escherichia coli microbial type culture collection-723 strain (E. coli MTCC 723) in water is reported. The sensor was constructed by functionalizing Ag-micro wire coupled with polyclonal antibodies of E. coli through self-assembled monolayers of cysteaminium. The biosensor detected the change in impedance caused by the presence of E.coli bacteria immobilized on Ag-micro wire electrodes. The antigen–antibody interaction was quantified by measuring impedance in the frequency range (1–100 Hz) at 10 mV applied potential. A dynamic range (102 colony forming units/mL (CFU/mL) - 108 CFU/mL) for E. coli was obtained with 10 min analysis time. The limit of quantitation of the developed biosensor and lower limit of detection is 102 CFU/mL. The proposed method is useful for sensitive analysis of E. coli in water samples.
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Keywords
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Water; Bacteria; Label-free detection; Electrochemical impedance spectroscopy; Silver wire; Immunosensor
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