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Sci. Lett. J. 2015, 4: 102
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Research Article
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Full Text
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Synthesis and characterization of ZnO-ZnAl2O4 whiskers and their application in biosensors
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Bhagaban Beheraa, Souvik Palb, Lizy Kanungob, Sunil Bhandb, Sudhir Chandraa
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a Centre for Applied Research in Electronics, Indian Institute of Technology, Delhi, New Delhi, 110016, India
b Biosensor Lab., Department of Chemistry, BITS, Pilani – K. K. Birla Goa Campus, Goa 403726, India
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Abstract |
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A simple and efficient technique to synthesize ZnO-ZnAl2O4 composite nano-whiskers has been reported. The application of developed nano-whiskers for sensitive bio-analysis and efficient bio-catalysis is demonstrated. For the synthesis of nanostructured ZnO-ZnAl2O4, Zn and Al in the form of thin films were taken as starting materials. To synthesize the composite nanostructured material, Zn thin film was deposited on oxidized silicon substrate using RF magnetron sputtering followed by Al film deposition by thermal evaporation. These layered metal films were thermally oxidized in a tube furnace at 400 - 550 ºC in atmospheric environment for 1 h. The structural and crystal phase analysis was done using Scanning Electron Microscopy (SEM) and X-ray Diffraction (XRD). The effect of temperature, Al thickness and time of oxidation on whisker growth has been studied. The composite nanostructure formation is attributed to Kirkendall effect. In this study, alkaline phosphatase (ALP) was used as biocatalyst immobilized on nanostructured ZnO- ZnAl2O4 composite. The novel biochip was tested for bio-catalytic activity for analysis of p-nitro phenyl phosphate and p-nitro phenol. The performance of the covalently immobilized ALP nano whiskers was found to be almost double over the physically adsorbed ALP.
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Keywords
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Zinc Aluminates; Thin films; Nanostructures; Whiskers; Biochip; Bio-catalysis
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